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3D Cell Culture Methods and Applications

Cell culture consists in growing cells in an artificial environment in order to study their behavior in response to their environment[1]. Different kinds of cell cultures can be found nowadays, and some would be more suited than others depending on its properties and applications.

Microfluidic for cell biology: Concepts and methodologies

Cell Biology: Microfluidic Concept and methologies for biologists to control the complete cellular microenvironment with microfluidics.

Microfluidic flow cells: off-chip pH monitoring for organ-on-chip

Microfluidic flow cells with embedded optical sensors, have been the most common choice for microenvironment monitoring like pH control.

3D cell culture: market and industrial needs

In this report, we identify the following two main purposes for the 3D cell market

Microfluidic cell culture – a beginner friendly review

Microfluidic cell culture is an innovative technology to improve classical lab protocols

Perfusion for live-cell imaging: A review

Live-cell imaging is a non destructive method which focuses on the observation of live cells and is widely used in cellular biology research and biomedical industry. This short review will present some technical aspects and challenges encountered in this field. Nowadays, the most widely used cell culture system is the static culture, where the cells are cultivated inside Petri dishes or multiwall plates. In static culture, the culture medium is supplied in a batch-wised manner.

Exploring Organ-on-a-Chip Technology: A comprehensive review

Microfluidic chambers for cell culture: Medium change

Ability to switch drugs in seconds or less in microfluidic chambers allows studying cell response at cells timescale.

Air-Liquid Interface and Optimized Cell Culture Substrates for Microfluidic Lung-on-a-Chip Applications

Explore the intricacies of air-liquid interfaces and optimized cell culture substrates in microfluidic lung-on-a-chip systems.

Organs on chip review

Multi-organs on chip could also allow us to witness the side effects of certain drugs on different organs, not limited to those that the treatment targets.

Perfusion for live cell imaging: Methods and techniques

This short note summarizes the methods and techniques employed to perform perfusion for live cell imaging and critical issues encountered.

Lab-On-a-Chip drug testing in Microfluidics

Microfluidics applications: A short review

Microfluidics is a term which appears more and more often in papers and scientific magazines; but, what exactly is microfluidics?

Acoustofluidics: Cutting-Edge Techniques for Microfluidic Particle Manipulation

Revolutionizing Microfluidics with Acoustic Particle Manipulation

Mechanosensing and Shear Stress in Microfluidics

Specifically, we will explore a mechanical force known as shear stress and its role in modulating cellular responses through a process known as mechanosensing.

Lab-on-a-chip 2024: review, history and future

A lab-on-a-chip is a miniaturized device that integrates onto a single chip one or several analyses, which are usually done in a laboratory; analyses such as DNA sequencing or biochemical detection.

Microfluidic Tumor-on-Chip Systems for Breast Cancer Research

Explore the advanced microfluidic tumor-on-chip systems revolutionizing breast cancer research. How these systems offer precise drug testing.

Perfusion for live cell imaging: Methods and Systems

Microfluidics for the diagnosis and treatment of tuberculosis

Imaging and microscopy: Perfusion chambers

The first live-cell imaging chambers were designed in the early twentieth century, shortly after mammalian cell culture techniques were developped

Organoids VS Organ-on-a-chip: A Comparative Review of Advanced In Vitro Models

Discover the transformative potential of organoids and organ-on-a-chip technologies in advancing drug testing, disease modeling, and personalized medicine

Liver-on-chip: keeping up with the technology

The liver is involved in more than 300 vital functions, but is mainly known for being part of the digestive tract, where it has the extremely important role of metabolizing both xenobiotics and nutrients (carbohydrates and lipids).

PDMS in biology research: A critical review on PDMS lithography for biological studies

PDMS advantages and drawbacks for cell biology are here discussed.

Sodium alginate and applications: a review

This review focuses on sodium alginate and its applications in microfluidics

Gradients generation for cell biology in microfluidics

Chemical gradients play a key role in many biological processes and regulate a number of cellular functions in vivo. Indeed, there are several examples of gradient-dependent phenomena in nature.

Microfluidics Devices in Pharmacogenomics Paving the way for precision medicine

Pharmacogenomics is the study of how an individual’s genetic variants influence drug responses and treatment efficacy.

Microfluidics in Cosmetic Research: Improved Formulations and Smarter Testing

Explore how microfluidics is revolutionizing cosmetic research from formulation to toxicology with ethical, precise, and scalable testing.

Lung-on-a-Chip: History, Origins and Development

While many animal models have been used to study lung diseases, they lack sufficient similarity with human systems, leaving gaps in what is possible in animal-based platforms.

Materials for microfluidic chips fabrication : a review 2017

Microfluidics involve different types of devices, and materials for microfluidic fabrication must be selected while bearing in mind all the requirements necessary for building a fine microfluidic device.

Gut-on-Chip: Advancing Intestinal Research

Discover how gut-on-a-chip technology is revolutionizing intestinal research & drug development by replicating the gut's complex environment.

Anti-aging research, microfluidics and organ-on-chips

Although we take part in various research projects such as artificial photosynthesis, pathogen detection and stem cell differentiation, the ultimate goal of our entrepreneurial adventure is to accelerate anti-aging research.

CRISPR-CAS9 and its Relation with Microfluidics

The integration of CRISPR-Cas9 with microfluidics has led to the development of innovative techniques for genetic editing and screening.

Organ-on-chip companies

Organ-on-chip companies developping innovative technologies

Microfluidics as a tool for drug delivery

Microfluidics, i.e. the science and engineering of fluid flows in microscale, can be the answer for a more effective and targeted drug administration.

Polydimethylsiloxane (PDMS) in Microfluidics: Properties and Emerging Smart Functionalities

Polydimethylsiloxane, called PDMS or dimethicone, is a polymer widely used for the fabrication and prototyping of microfluidic chips. It is a mineral-organic polymer (a structure containing carbon and silicon)

Magnetic particle separation : a short review

This review presents a few examples of applications where magnetic flux sources, magnetic particles and microfluidics are combined in order to perform particle sorting and handling.

Organ-on-a-chip

Since 2012, more and more people, companies or lab, have worked on the organ-on-a-chip. These cell cultures can, thanks to microfluidics, mimic the cells microenvironment of the human body. Thus, these chips could become wonderful search accelerators and we can hope that, in ten years, they could replace the animal testing. Finally, organs on chips could lead us to personalized medicine.

History of Microfluidics

Today, MICROFLUIDICS is a distinct and major technological field, but 20 years ago it was not like this and its boundaries were not so well defined. The history of microfluidics is strictly related to several other areas

Particle encapsulation – from particle synthesis to encapsulation methods

Finding the right technique for particle encapsulation using micro and nanoparticles is key for a successful particle encapsulation protocol.

A Review of heating and temperature control in microfluidic systems: Techniques and Applications

This review presents an overview of the different techniques developed over the last decade to regulate the temperature within microfluidic systems.

Microreactors & Microfluidics in Chemistry – a Review

This review introduces the field of microfluidics and provides an overview of the advantages, disadvantages, and current applications of microfluidics in chemistry.

The Dynamics of Fungal Spore Dispersal: Insights from Microfluidic Models

Microfluidics: A general overview of microfluidics

Microfluidics is both the science which studies the behaviour of fluids through micro-channels and the technology of manufacturing microminiaturized devices containing chambers and tunnels through which fluids flow or are confined.

Recent research breakthroughs in lung-on-chip technology

It could be extremely interesting to build a human-on-chip that will model the interactions between different organs, but it is also essential to develop simulations of tissue-tissue interfaces and more generally of local organ behavior.

thermoelectric sensor

Introduction to thermoelectric sensor | Microfluidics immunosensors offer multiple advantages over the conventional immunoassays that include improved reaction rate, reduced time for incubation of the reactants, and decreased reagents and sample consumption. Moreover, miniaturization and integration of the multiple assay components permit automation, precise flow control, increased reproducibility, and the possibility for high-throughput analysis.

High accuracy microfluidic pumps

High accuracy microfluidic pumps are important for many applications from dynamic cell culture to droplet or nanoparticle generation.

Heart-on-chip

A heart-on-chip is a microfluidic chip reproducing the mechanisms of a heart, in order to test medicine quickly and observe the reaction of heart cells. Great care is given to mimic the mechanics of a heart in an artificial structure, lined with live heart cells.

Coronavirus (SARS-CoV2) diagnostic with microfluidics

Coronavirus (SARS-CoV2) diagnostic through microfluidics

Circular channel fabrication in microfluidic devices

Microfabrication techniques for a circular channel

PDMS: A review

Polydimethylsiloxane, called PDMS or dimethicone, is a polymer widely used for the fabrication and prototyping of microfluidic chips.

Microfluidic “mini-brains”: keeping up with the brain-on-chip technology

At the beginning of the third millennium, due to prolonged aging, neurodevelopmental disorders are growing and a much deeper understanding of the brain is necessary.

Air bubbles and microfluidics

Air bubbles are among the most recurring issues in microfluidics. Because of the micrometric dimensions of the tubes and channels

Microfluidics Definitions

Microfluidics: definitions. This short review aims to cover the main microfluidics definitions from the simple word by word science to the advantages & applications

Chemical resistance of microfluidic materials

One of the key criteria to choose your microfluidic device material is its chemical resistance. This review will help you choose one depending on your application.

Microfluidic Spheroid Culture Platform

Microfluidics for point-of-care diagnostic devices: a review

This review focuses on point of care (POC) diagnostic devices for pathogen detection

Use Cases 14 pages found

Endothelial Cell Culture

Microfluidic Particle Sorting

Blood-brain barrier on a chip

Gut on a Chip

Recirculation Loop setup

Microfluidic Flow Cytometry

Giant unilamellar vesicle (GUV) production

Drug screening

Fluid Concentration Gradient Generation

Automated Sampling Solution for Continuous Analysis

High Precision Volume Sensor

Microfluidic Chemotaxis Assay

Microfluidic liquid-liquid extraction

Application notes 43 pages found

Automated Cell-Seeding for Dynamic Cell Culture

A simple guide to do dynamic cell culture by automating cell seeding in a microlfuidic chip

Mechanoactivation of Single Stem Cells in Microgels Using a 3D-Printed Stimulation Device

A microfluidic and pressure-driven platform enabling reproducible mechanoactivation of individually encapsulated stem cells under live-cell imaging in a true 3D environment.

Cell-cell interactions on chip

This application note describes how co-culture of different cell types in separate but interconnected chambers is possible in a microfluidic platform

How to stain cells cultured in a microfluidic chip?

In this application note we describe how to stain cells for dynamic cell culture with different microfluidic setups.

Cell biology

Microfluidics is widely used to develop tools for cell biology. The micrometer scale of microfluidic devices is particularly adapted to work with cells.

Microfluidic perfusion for dynamic cell culture

In this application note we describe how to do cell perfusion for dynamic cell culture and a way to enable uni-directional recirculation of medium.

Medium recirculation for dynamic cell culture

In this application note we describe how to create a medium recirculation for dynamic cell culture with a microfluidic setup.

Microfluidic cardiac cell culture model

This application note proposes a microfluidic cardiac cell culture model (μCCCM) to recreate mechanical loading conditions observed in the native heart (in both normal and pathological conditions) by using an Elveflow OB1 pressure and flow controller.

Performing live cells assays on chip

This application note presents how to perform cell culture on chip using the Cell and Biology Pack and the MicroSlides developed by ALine Inc.

Medium recirculation using microfluidic valves

In this application note we describe how to set up medium recirculation by using microfluidic valves

Microfluidic Perfusion medium switch using IBIDI© chips

Medium switch is widely used in cell biology. One application is the study of cell behavior under given flow conditions for different samples. In this tutorial, we walk you through the steps of a fast and stable medium switch using IBIDI© flow cells.

Cross flow membrane for molecular transport

Study the impact of molecular transport on cell cultures with a cross flow membrane chip and microfluidic instruments.

Pressure-driven flow control

This application note explains how pressure-driven flow control works, the advantages & disadvantages of the different technologies, and the technical choice to make to how to perform effectively your microfluidic experiment.

Sequential Production and Trapping of Droplets

Microfluidic sequential production and trapping of droplets for in situ optical analysis.

Stimulation of C. elegans mechanoreceptor neurons using microfluidics [Publication Recap]

This article covers the work of Adam L. Nekimken et al. published in Lab Chip in January 2017. The research team introduced a novel microfluidic device that enables high-resolution imaging of cellular deformations in response to precise mechanical stimuli to the surface of the C. Elegans worm cuticle.

Biofilm testing under flow

Biofilm testing using a simple microfluidic chip channel for in situ observation of their development under flow conditions.

Fluid recirculation – Perfusion microfluidic system

The MUX Inj is a bidirectional 6-port / 2 position valve allowing to perform switches between two setup configurations. One application is to make a fluid recirculation set-up with a flow rate always going through the chip in the same direction. In this application note, we walk you through the steps of setting up a unidirectional recirculation through a semi permeable membrane.

Controlled medium switch on a microfluidic chip

Changing the injected liquid inside a microfluidic device has numerous applications, such as sequential sample injection for biochemistry and flow chemistry, or medium switch for cell biology and 3D cell culture on chip. The easiest solution is to replace the liquid injected, but it is often not possible:

Weak protein interactions quantified using a microfluidic pump [Publication recap]

This article covers the development of an experimental method enabling Shahar Sukenik et al. to study protein interactions and detect the dissociation of GAPDH and PGK proteins in order to quantify their stoichiometry directly inside the cell by modulating the cell-volume

How to choose flow regulation parameters?

Flow regulation is a compulsory operation in most of the microfluidics operations. In some applications such as 2D or 3D cell culture, flow regulation is essential since accurate micro-environmental parameters control is required. Elveflow do it’s best to make this operation as easy as possible to help you to focus on what really matter in your setup.

Exploring Pneumatic Pumps and Pressure-Driven Flow Control Technology

This application note explores the basic principle of pneumatic pumps and a flow controller based on the basic principle of pneumatic pumps, known as pressure driven flow control. It also demonstrates the applications of pressure driven flow control in a range of industrial & research fields.

Rheodyne microfluidic valve bidirectional ten-way injection

Maintain a controlled flow rate? This application note aims to help you maintain a controlled flow rate during a flow line switch thanks to the Mux Distributor. This valve allows to switch between up to 10 lines to inject several fluids sequentially in your system. It has many applications such as sequential sample injection for biochemistry and flow chemistry, or medium switch for cell biology on chip.

How to study bacteria by microfluidics

This application note explains how to study bacteria adaptation to stress and environmental changes such as antibiotics.

Microfluidic calculator by Elveflow | Application note

Elveflow developped a free online microfluidic calculator. This tool has been designed to help researchers and especially non specialists of the microfluidics field. It helps you assess key parameters to configure your microfluidic experiment.

Circulating Tumor Cells entrapment using microfluidics

Prostate cancer is the second leading cause of cancer-related death for men. Circulating tumor cells (CTCs) are considered as a marker of early cancer diagnosis and disease severity. Their screening in blood is thus crucial to detect metastatic stage in cancer patients.

Controlled microfluidic sample injection

The MUX inj is a bidirectional 6-port / 2 position valve allowing to perform switches between two set-up configurations. One application is to perform a stable and unidirectional fluid recirculation or to inject a precisely controlled volume of drug. This application note focuses on the example and will walk you through the steps of a successful experiment.

Zero Flow microfluidic experiment

This application note describes how to generate a controlled flow inside a microfluidic chip and stop it completely (zero flow) by using an Elveflow® pressure control instrument (OB1) and valves.

Microfluidic Droplets on demand

Active droplet generation in microfluidics is of high interest for a wide range of applications. It provides an additional degree of freedom in manipulating both the size and the formation frequency of micro-droplets. This additional control is extremely desirable for complex operations which rely on the accurate control of both parameters.

Instantaneous stop flow using flow multiplexer

Because of fluidic compliance of tubing and chip, achieving stop flow into a microfluidic device remain a challenge with conventional setup. One solution to achieve stop flow in hundreds of milliseconds into a microchip without residual flow is to use a pressure controller coupled with flow switch.

Microfluidic controlled drug switch

This application note shows how to easily perform controlled drug switches on an hypothetic chemical or biological environment (drug screening or cell culture) with an Elveflow® MUX as shown on these pictures:

How do you synchronize a microscope with a microfluidic perfusion system?

This application note describes how to combine and synchronise liquid perfusion and imaging using an Olympus spinning disc confocal microscope together with an Elveflow pressure-driven flow controlled microfluidic system.

Tissue engineering: Heart Ventricule assisted by a microfluidic pressure pump

Until recently, microfluidic devices have been employed to support tissue-engineering experiments on basal lamina, vascular tissue, liver, bone, cartilage and neurons as well as organ-on-chips.

Microfluidics for microscopy imaging and treatment of Arabidopsis thaliana primary roots in vivo

Microfluidics for microscopy imaging in plant biology allows to observe, in vivo, the biological response of plant roots to various stimuli.

Peristaltic pump compared to pressure driven flow control

In this review we will explain the operating principle of pressure driven flow control, the advantages / disadvantages of the different technologies and how to choose between a peristaltic pump and pressure control depending on your requirements.

Calculate flow rate microfluidics

Elveflow's microfluidic calculator permits to calculate flow rate in microfluidics dependent upon the microfluidic system from the device, the tubing to your fluid properties.

Microfluidic resistance calculator

Elveflow's developped a microfluidic resistance calculator dependent upon the microfluidic system from the flow rate, the device, the tubing to your fluid properties.

viscosity conversion

The application note describes how to convert various units of viscosity from one to another: viscosity conversion from Poise, Pa.s, Dyn.s/cm²...

Shear stress calculator

The application note describes how to convert various units of shear stress and/or pressure from one to another: shear stress conversion from Pascal, atmosphere, and N/m²...!

Using the perfusion pack to perform fast medium switch

In this application note, we will describe how to perform an automated and fast medium switch thanks to the Perfusion Pack.

How to handle monodisperse alginate droplets & beads production with microfluidics

Highly monodispersed alginate beads can be easily generated with a microfluidic droplet generation system.

How to perform single spore encapsulation

This application note will show you how highly monodispersed droplets can be easily generated to encapsulate single spore of fungus using a microfluidic droplet generation system.

Fluorescence reader for microfluidic qPCR

Fluorescence reader for microfluidic qPCR: faster, more sensitive and less expensive than most optical microscopes, it is a smart alternative for real-time fluorescence measurements of your on-chip qPCR signal.

TERMIS EU 2025 Highlights with Elveflow

Antifungal screening on chip using droplet based microfluidics

Droplet-based microfluidics and single spore encapsulation offer the key for a breakthrough in antifungal screening and fungicide discovery.

Blogs 17 pages found

MPS World Summit 2025 Highlights with Elveflow

Selectbio 2024 California Highlights with Elveflow

Pressure-Based Flow Controllers: Precision and Versatility for Microfluidic Applications

MicroTAS Adelaide 2025 Highlights with Elveflow

Revolutionizing Drug Testing: The Role of Microfluidic Technologies in Complementing Traditional Methods

Liquid Handling in Microfluidics: Precision & Automation with Elveflow

Biocompatible Polymers in Microfluidics: Applications & Insights

Tumor Microenvironment Signaling and Therapeutics in Cancer Progression

Pressure Sensor Applications: Explore Elveflow’s High-Precision Solutions

GDR MicroNanoFluidique and GDR Réparer l’Humain Highlights with Elveflow

Decoding the Tumor Microenvironment: Cancer Progression and the Role of Microfluidics

Exploring Bacterial Adaptation: An Interview with Dr. Bianca Sclavi

Syringe Pumps: Precision and Efficiency in Microfluidics

Elveflow Japan Trip 2024

Peristaltic Pump vs. Pressure-Based Flow Control Systems

Thermoplastic Chips for Biomedical Applications Dr. Hugo Salmon Insights

Pages 16 pages found

Microfluidics for cell biology
Microfluidic Biology Packs
test product 2
Molecular and cellular medicine UKRI Grant
Organs-on-Chip and 3D models
Organs-on-chip and 3D cell culture
Microfluidic calculator
Microfluidic testimonials
Microfluidics reviews
Elveflow company history: a journey in microfluidics
Partners
Microfluidic-cell-culture
Microfluidics for cell biology
Biology Packs
Microfluidics research
Elveflow newsletter: Get your Microfluidics Report!

Research summaries 12 pages found

Dynamic single cell screening

The authors describe the use of a dynamic dual micro-valve system, which can be used to screen cells based on their size and print single-cells into separate cell culture chambers.

Mechanical cell compression – a short review

Design, fabrication and application of microfluidics-based flexible microdevices to apply mechanical cell compression and stimulation

A method to dynamically screen and print single cells

The study explores a method to dynamically screen and print single cells using microfluidics with pneumatic microvalves.

Microfluidic Constriction Assay Reveals Fibrotic Stiffening in a Spheroid Model of Myocardial Ischemia

Discover how a microfluidic constriction assay in large cardiac spheroids replicates myocardial ischemia and fibrotic remodeling to advance cardiovascular research.

USING GUT-ON-A-CHIP AND 4D IMAGING TO UNDERSTAND PATHOGEN INVASION

Discover how mechanical cues influence pathogen-host interactions, and the challenges in capturing these in real-time microscopy.

Biocompatible Micron-Scale Silk Fibers Fabricated by Microfluidic Wet Spinning

The authors developed, tested and validated a method that applies a pressure-driven flow controller to fabricate biocompatible micron-scale silk fibers by microfluidic wet spinning.

Development of a human bladder on chip model

This review article explores the functioning of a human bladder on chip model to study the key features of bladder physiology that are important to early UPEC infection.

On-chip particle manipulation

This short review presents the fabrication and testing of a 3D microfluidic micromixing device to perform on-chip particle manipulation.

Electrokinetic sandwich assay and DNA mediated charge amplification

Multicomponent laser printing

Thus far, 3D laser-micro and nanoprinting offered a widespread alternative to prepare complex but single constituent 3D microfluidic structure. This work proposes a system based on a microfluidic chamber integrated into a state-of-the-art laser lithography apparatus that allows the use of several materials to 3D print complex structures in the most effective manner.

Unravelling two-phase flow and CO2 storage in geological reservoirs

This article investigates the behaviour of immiscible two-phase flow through porous media focusing on how pore-scale processes impact macroscopic fluid front behaviour.