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[caption] => Fig 2. Alginate microparticles produced using microfluidic technique.
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[texte] => This review focuses on sodium alginate and its applications in microfluidics
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[title] => Sodium alginate and applications: a review
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[part_1] => Alginate beads generation pack
[part_2] => Alginate beads generation
[part_3] => Alginate beads generation system straight out of the box! All you need to know to perform hydrogel particle production!
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[titre] => Reproducible & high monodispersity
[texte] => Produce monodisperse hydrogel beads (PDI <5%)
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Alginate beads production has never been that easy using our all-in-one hydrogel particle generation system !
Based on our high accuracy OB1 flow controllers , this all-in-one solution contains all the required parts for researchers to set up their own alginate beads generation system out of the box. It brings the many benefits of microfluidics , including excellent monodispersity , sphericality, reproducibility, and high throughput compared to other hydrogel particle production techniques.
A standard alginate beads generation pack contains one pumping channel to flow the aqueous alginate phase and another pumping channel to push the continuous oil phase through our droplet generation chip, enabling the generation of alginate droplets in oil.
The droplet size will be determined by the chip channel size and the flow rate ratio of both phases. Flow rates can be measured thanks to our multiple flow rate sensors (MFS or BFS series).
For the detailed experimental steps, please refer to our application note !
To help you determine your flow rate, pressure to apply, the best tubing resistance length for your setup, wall shear stress for biology applications, cell culture, and many more…
Elveflow provides its microfluidic calculator dedicated application notes :
Publications
Droplet microfluidics as a tool for production of bioactive calcium phosphate microparticles with controllable physicochemical properties, P. Habibovic et al., Acta Biomaterialia (2021).
3D flow-focusing microfluidic biofabrication: One-chip-fits-all hydrogel fiber architectures, Rui L. Reis et al. , Applied Materials Today (2021).
Additive batch electrospinning patterning of tethered gelatin hydrogel fibres with swelling-induced fibre curling
Elisabeth L. Gill, Wenyu Wang, Ruishan Liu, Yan Yan Shery Huang, Additive Manufacturing, 2020, Volume 36, 101456, DOI: 10.1016/j.addma.2020.101456
Highly Ordered Gelatin Methacryloyl Hydrogel Foams with Tunable Pore Size
Friederike Dehli, Lisa Rebers, Cosima Stubenrauch, and Alexander Southan, Biomacromolecules 2019 20 (7), 2666-2674 DOI: 10.1021/acs.biomac.9b00433
The amazing benefits of microfluidics can be applied to many droplet applications and therefore the content of the alginate pack can be adjusted to suit your specific needs. Contact us for more details.
Options
Additional pumping channels
Additional flow rate sensors
Computer
Microscope and camera
Why choosing microfluidics for alginate beads generation?
Microfluidics brings reproducibility and control over particle or droplet size that is not achievable by any other technique. Continuous hydrogel beads generation monodisperse particles (PDI < 5%) .
Moreover, microfluidics is an ideal technique for handling ultra small volumes and control what goes into each droplet or bead. Microfluidics give you flexibility to easily generate identical carrier with the same content . For more detailed protocole about alginate beads generation, please refer to our application note !
1. Select the right parameters to obtain the desired beads size Elveflow offers a specific chip that allows you to generate a broad of range of beads size, shape and materials. For more information, please refer to our application note about alginate beads generation.
If you’re unsure about the settings that are best suited for your application, get in touch with one of our experts!
2. Choose your reservoir size Are you working with small samples or are you looking into manufacturing 100x mLs of hydrogel beads solution? Elveflow offers a comprehensive range of reservoirs compatible with our OB1 flow controller , from 1.5 mL Eppendorf tubes to 100mL bottles.
3. Oil & surfactants To make monodisperse and stable alginate beads, Elveflow offers a range of oils and surfactants. For more detailed information, please refer to this application note !
Interested in highly monodisperse hydrogel beads production?
Hydrogel-based delivery systems are finding more and more applications, from encapsulation , protection, to the release of bioactive agents in many fields such as pharmaceutical , supplements , food industries , for cell/bacteria/microbes culture & implantation, and cell-based genes in biological research.
Are you interested in implementing hydrogel beads production in your lab?
Contact us to have your dedicated your dedicated hydrogel beads generation pack that suits your application needs!
Highly monodispersed alginate droplets, size in the 50µm range. Experiment results by the Elveflow team.
Encapsulate your cells in alginate beads for fluorescence activated cell sorting
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[texte] => MICROCOLONIES IN ALGINATE BEADS FOR FACS SORTING SETUP An all-in-one pack guarantees a good compatibility between the different instruments, allows you to start your experiment right away, is piloted by a single software and can be used for other different applications. These are a few arguments why a pack is the easiest way to setup a microfluidic experiment.
A TYPICAL PACK CONTAINS:
OB1 flow controller (Elveflow) with at least two channels 0/2000 mbar
2x flow sensors MFS 0/7 µL/min (Elveflow)
Kit starter pack luer lock with fittings and tubings
2x 15 mL Falcon reservoirs
Microscope for observation (optional)
ESI control software (automated sequences possible) (Elveflow)
Our setup allows you to entrap cells in alginate beads. Once incubated, they grow into microcolonies for fluorescence activated cell sorting.
You can also use the setup for single cell screening as well.
THE CHIP USED IN THE SETUP
The chemicals used for sample preparation:
Alginate (low viscosity)
Acetic acid – Merck, SKU: 33209-1L-M
HFE7500 oil with 2% surfactant
Droplet breaking solution
1 vial of droplet oil
1. Davies, D. (2012) Cell separations by flow cytometry. Methods Mol. Biol. 878, 185−199.
2. Weaver, J. C., Bliss, J. G., Powell, K. T., Harrison, G. I., and Williams, G. B. (1991) Rapid clonal growth measurements at the single-cell level: gel microdroplets and flow cytometry. Nat. Biotechnol. 9, 873−877.
3. Sahar, E., Nir, R., and Lamed, R. (1994) Flow cytometric analysis of entire microbial colonies. Cytometry 15, 213−221.
4. Zengler, K., Toledo, G., Rappe, M., Elkins, J., Mathur, E. J., Short, J. M., and Keller, M. (2002) Cultivating the uncultured. Proc. Natl. Acad. Sci. U. S. A. 99, 15681−15686.
5. Duarte, J. M., Barbier, I., & Schaerli, Y. (2017). Bacterial microcolonies in gel beads for high-throughput screening of libraries in synthetic biology. ACS synthetic biology, 6(11), 1988-1995.
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It brings the many benefits of microfluidics, such as excellent monodispersity , reproducibility and scalability to your daily work in order to achieve great science.
It has never been easier to start generating droplets following a step-by-step User Guide and quickly master the process with the same system.
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An all-in-one solution for droplet-based microfluidics
Do you want to implement droplet-based microfluidics? We designed a comprehensive solution based on our high precision instruments set. This Pack includes all you need to understand the droplet generation process from 0 to 1.
Get the Pack
Unbox the instruments
Install the setup following our step-by-step User Guide
Start generating your first droplets thanks to detailed protocols.
Start from scratch and quickly master all key parameters triggering and controlling droplet generation.
Droplet forming inside the microfluidic chip
Benefit from the highest standards of microfluidic instruments
Moreover, it is an ideal technique to handle ultra small volumes and control droplet generation precisely. It give you flexibility to easily generate identical droplets with the same content or to create individual droplets with unique payloads.
This Droplet Generation Pack includes our OB1 flow controller best-seller.
Thanks to the performance of this instrument, you will be able to generate:
Droplets ranging from 10 to 80µm diameter (and more using alternative microchips)
Highly monodisperse droplets (CV<3%)
Overview of the droplet pack setup
We can provide you with two Droplet Pack versions : the small version , to generate smaller droplets ranging from 10 to 40µm and the large version , for droplets ranging from 50 to 80µm diameter. Each Pack is composed of:
One OB1 flow controller with 2 control channels (one for each phase)
Two flow rate sensors (one for each channel)
Two fluidic resistances (for stable and accurate flow control)
Two Microchips from ChipShop, a leading microfluidic chip manufacturer.
All necessary reservoirs and tubings.
User guide droplet generation pack
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Application notes
Emulsions generation & stability study
Synthetic Organelles
Nanoparticles & nanohydrogels
Microgels
Microbubble generation
Liquid foam templating
Single Cell Encapsulation
Encapsulation
Drug-delivery
Microfluidic Droplets on demand
Alginate beads production
Droplets in microfluidics capillary
Microfluidic flow focusing droplet generation
All our application notes
Reviews
Introduction to droplet-based microfluidics
Digital microfluidics using pressure driven flow
Digital microfluidics: microfluidic droplets & emulsion science
What is digital microfluidics?
Researchers’ opinion on droplet generation in microfluidics: syringe pumps or pressure control?
Droplet Sequencing (Drop-Seq)
Droplets encapsulation for biological applications: a review
Droplet detection and measurement in microfluidic channels
Droplet production methods
Sodium Alginate and applications: a review
All our reviews
Publications using our equipments
Image-based closed-loop feedback for highly mono-dispersed microdroplet production, Crawford et al, Scientific reports (2017)
High magnetic field gradient particle separation for biomedical applications. Conference Poster, Lennart Göpfert (2021).
Droplet microfluidics as a tool for production of bioactive calcium phosphate microparticles with controllable physicochemical properties, P. Habibovic et al., Acta Biomaterialia (2021).
3D flow-focusing microfluidic biofabrication: One-chip-fits-all hydrogel fiber architectures, Rui L. Reis et al. , Applied Materials Today (2021).
Thin film drainage dynamics of wheat and rye dough liquors and oat batter liquor, F. Janssen et al., Food Hydrocolloids (2021).
Coalescence Dynamics in Oil-In-Water Emulsions at Elevated Temperatures, B. Bera et al., Scientific Reports (2021)
Size-dependent particle migration and trapping in three-dimensional microbubble streaming flows Andreas Volk et al., Physical Review Fluids (2020).
High-Throughput Step Emulsification for the Production of Functional Materials Using a Glass Microfluidic Device Macromolecular Journals, D. Weitz et al.
Controllable generation and encapsulation of alginate fibers using droplet-based microfluidics Lab on a Chip, Nov 2015, A. J. deMello et al.
Image-based closed-loop feedback for highly mono-dispersed microdroplet production Scientific Reports, Sept 2017, G. Whyte et al.
Negative Pressure Induced Droplet Generation in a Microfluidic Flow-focusing Device Analytical Chemistry, Feb 2017, Say Hwa Tan et al
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Content of the pack
1 x 2-channel flow controller (OB1-2200) 2 x flow rate sensors
Small version : 1 x MFS-D-2 (aqueous phase) & 1 x 2 x MFS-D-3 (oil phase)Large version : 2 x MFS-D-2 (aqueous / oil phase)
2 x flow resistances
Small version : 1 x 22-H (aqueous phase) & 1 x 23-I (oil phase)Large version : 1 x 22-H (aqueous phase) & 1 x 22-I (oil phase)
2 x ChipShop microfluidic droplet chips Reservoirs and holder
6 x reservoirs with their pressurized caps (2 x 50mL; 2 x 15 mL; 2 x 1.5 mL)
1 x quick connect reservoir holder (Wall-E holder)
And more!
All necessary accessories: tubing, connectors, filters, etc…
1 x 10 mL vial of droplet oil (HFE-7500)
Control and automation software (Elveflow Smart Interface)
1 x User Guide which includes:
Detailed contents list
Step-by-step installation guide
Detailed protocol to generate droplets with the Pack
Screenshots and pictures to get familiar with our equipment.
Two versions available depending on the desired droplets
Our two versions only differ by the flow rate range of the oil phase.
The small version includes a MFS-D-3 allowing perfect feedback loop control of the flow rate to generate droplets ranging from 10 to 40 µm diameter.
The large version includes a MFS-D-2 allowing perfect feedback loop control of the flow rate to generate droplets ranging from 50 to 80 µm diameter.
Important note: our products are all intercompatible and you may acquire additional flow rate sensors or add extra products for specific applications. You will still benefit from the centralised ESI software control.
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Droplet-based microfluidics
Droplet-based applications
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Do you know how to implement droplet-based microfluidics?
Are you aware of how to compute shear stress and microfluidic resistance in your system?
Elveflow developped a microfluidic calculator
To help you determine your flow rate, pressure to apply, the best tubing resistance length for your setup, wall shear stress for biology applications, cell culture, and many more…
Elveflow provides its microfluidic calculator dedicated application notes :
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A well-chosen microfluidic chip
The microfluidic chip is where the two immiscible phases (water and oil here) are precisely injected and mixed to generate monodisperse droplets (CV < 3%). The two microfluidic chips are made of Topas. Topas is a cyclic olefin copolymer (COC) resin which is a chemical relative of polyethylene and other polyolefin plastics.
[texte_2] =>
A reservoir holder with 2 push-in connectors
Are you working with small samples or are you looking into manufacturing 100x mLs of emulsion? Elveflow offers a comprehensive range of reservoirs compatible with our OB1 Flow Controller, from 1.5 mL Eppendorf tubes to 100mL bottles.
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Enhanced flow resistor systems
Flow resistances are used to increase the resistivity of the microfluidic system to improve the stability and control of the flow rate in the system.
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Simple and intuitive instructions are provided to quickly and easily make droplets and control droplet generation parameters. When starting out, the user can follow step by step the provided protocol to obtain droplets of the specific size. In a second stage, the user can rely on the numerous tips provided and explore the “going further” section to complete its training in droplet generation and microfluidic flow control.
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[texte] => Generate organoids for drug screening or regenerative medicine
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[titre] => Mimic tissues & organ's features
[texte] => Reproduce key biological features to study drug responses
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Organoids and spheroids are three-dimensional structures composed of self-assembled cells that mimic the characteristics of tissues and organs. These structures are typically grown by culturing cells under non-adherent conditions that allow them to self-organize into these complex structures [1] . A current challenge is to integrate cellular complexity into organoids in a controlled manner, leading to organized assembly and takeover of tissue function [2] .
Using microfluidics for organoids or spheroids in droplets:
provides an environment with different physicochemical properties, such as stiffness or permeability, which can influence organoid formation and function allows a wide variety of polymers to be be used for the dispersed phase is ideal for generating high throughput and reproducible organoids or spheroids the precision and composition of droplets, which are critical for uniform and reproducible organoid formation
Elveflow offers you a versatile computer-controlled microfluidic setup to enable organoid & spheroid encapsulation in an efficient manner.
ORGANOID & SPHEROID ENCAPSULATION PACK: THE PRINCIPLE One technique for creating organoids and spheroids is to encapsulate cells in droplets of a hydrogel, such as alginate or agarose, using microfluidic devices. The droplets act as small bioreactors, providing the cells with the necessary nutrients and oxygen for growth, while also allowing for the exchange of waste products.
ORGANOID & SPHEROID ENCAPSULATION PACK: THE SETUP
This pack is composed of the premium Elveflow product line and our best-seller, the OB1 flow controller . Thanks to the high performance of this equipment set, you will be able to:
produce robust and highly monodisperse emulsions (CV<3%) Fast and efficient mixing of reagents for droplet generation have access to all the necessary elements to encapsulate cells easily provide ultra-precise pressure-driven flow control generate 10 to 80 µm diameter droplets (and more depending on your microchip)
The solution can be configured as per the user’s requirements. Our experts can help you define the best possible setup to reach your goal.
Thanks to the versatility of the instruments, and the upgradability of our range, Elveflow’s solution can be adapted to your needs.
A TYPICAL PACK CONTAINS: As proof of concept, you can consult this Nature paper on Tumour-on-chip microfluidic platform for assessment of drug pharmacokinetics and treatment response
References
[1] Kratochvil, M. J., Seymour, A. J., Li, T. L., Paşca, S. P., Kuo, C. J., & Heilshorn, S. C. (2019). Engineered materials for organoid systems. Nature Reviews Materials, 4(9), 606-622 .
[2] Takebe, T., & Wells, J. M. (2019). Organoids by design. Science, 364(6444), 956-959.
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A TYPICAL PACK CONTAINS:
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Beta Packs, such as the Dielectrophoretic Sorting Pack pack, are an assembly of instruments that include Elveflow’s catalog products and, at least, one beta instrument. The beta instrument has recently been developed by our Innovation team, and it is in testing phase. If you are interested in this beta pack, its applications, or possible variations to better fit your needs, contact our experts !
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[question] => Can I order a Beta Pack?
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Our Beta Packs, such as the Dielectrophoretic sorting are available under specific conditions. As these packs are still in the developmental stage, we have some eligibility criteria to ensure their optimal success rate. A consultation with our experts is necessary to evaluate your eligibility to our Beta Testing Program.
At Elveflow, we believe in tailoring our approach to suit each researcher’s unique needs. Therefore, before purchasing a Beta Pack, we encourage you to reach out to our technical team for a detailed discussion of your research objectives and requirements.
To learn more about our Beta Packs or to schedule a consultation with our experts, please contact us at innovation@elvesys.com . We look forward to collaborating with you and supporting your research endeavors.
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Elveflow’s
Starter Pack can be set up in many ways that bring the many benefits of pressure-driven pumping to your microfluidic applications. One of the key advantages of Elveflow’s
OB1 Flow Controller is to remove any flow oscillations and enable very fast flow rate changes (no inertia) while remaining extremely stable.
From simple single-channel microfluidic flow to multi-channel droplet experiments, the Starter Pack covers hardware needs for a wide range of applications. The whole system is controlled by the powerful ESI software which allows you to simply set pressures and monitor your experiment, or try advance functions such as full automation and running scripts.
You can add different Elveflow modules to suit your experimental needs. These modules include valves, sensors, bubble traps and many more.
Experiments performed with the microfluidics Starter Pack
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APPLICATION notes
And many more depending on your field of application:
To go further in fluid handling:
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Microfluidics can be applied to lots of varied applications and therefore the content of the Starter Pack can be tweaked to suit your specific needs.
Content A standard starter pack contains the following:
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Why use microfluidics?
Microfluidics is the science of manipulating and controlling fluids, usually in the range of microliters (10-6 ) to picoliters (10-12 ). It is an amazing technology that brings lots of benefits to many varied applications areas of science such as:
Very low sample and reagent consumption High reproducibility Upmost control of experimental conditions (temperature, mixing, pressure, etc…) Easy automation Continuous processes And many more!
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Control flow rates directly Pressure-driven flow controlled microfluidics : Switch from an accurate pressure control to an effective flow rate control by adding Elveflow’s Flow Rate Sensors.
Those sensors, combined with the ESI software and its built-in feedback loop control, enable monitoring and control of liquid flow rates. You can dial in a flow rate value and your system will quickly and accurately reach that flow rate by adjusting the system pressure.
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Elveflow offers a variety of chips designed for specific applications such as droplet generation, flow focusing, cell encapsulation, and many more.
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Upgrade your OB1 Flow Controller The OB1 Flow Controller is a powerful instrument that can be tailored to suit your needs. We offer different channels, for vacuum and/or pressure, from -900 mBar up to 8000 mBar. You can swap or add channels to your OB1 Flow Controller at a later date. Contact us to find out more about the procedure.
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The OB1 allows you to control the output pressure of up to 4 channels independently, from -900 mbar to 8 bar, for a wide variety of advanced microfluidic applications.
Thanks to our proprietary Piezoelectronic Technology, the OB1 MK3+ is 10 times more stable and up to 10 times faster than other microfluidic flow controllers.
What is a microfluidic pressure-driven flow controller?
Cutting-edge Microfluidic Flow Controller
Designed by scientists for scientists, the versatile and powerful OB1 MK4 pressure controller provides the perfect flow control for all kinds of applications.
The best performance on the market: Piezoelectric Technology The Elveflow OB1 MK4 is one of the best performing microfluidic flow control instruments worldwide that uses piezoelectric regulators. The piezoelectric technology gives you 20 times more precise and 10 times faster flow control than any other flow controller on the market.
Customizable & upgradable: 1 module, up to 4 channels, 5 pressure & vacuum ranges available The OB1 MK4 can be configured according to your needs. In one piece of equipment, you can have up to 4 pressure and/or vacuum channels (and other customized options).
Get the fastest flow rate control when paired with a flow sensor Connect the OB1 MK4 to a standard liquid flow rate sensor MFS )our premium Coriolis flow sensor BFS , suitable for both liquid and gas)
Full control software, SDK, and UART communication Single and intuitive software to get started in a few clicks and automate complex and long experiments.
OEM version available The OB1 MK4 can be used on a bench setup or embedded in your own product.OB1 MK4 OEM solution.
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[texte] => Applications of Pressure-Driven Flow Control
Thanks to its ultra-fast response time, high stability, and precise flow control, the OB1 pressure controller is a preferred choice for a wide range of liquid handling microfluidic applications, compared to syringe pumps or peristaltic pumps. Combined with precision flow sensors (like MFS or BFS ), the OB1 pressure controller can accurately control flow rate in any microfluidic setups. Below is an overview of how pressure-driven flow control improves research across different domains.
Droplet & Bead Generation
Droplet Microfluidics Flow control microfluidics offers unmatched stability and responsiveness when generating monodisperse droplets in microchannels. The precise flow control is essential for the generation of identical droplets avoiding most post-synthesis steps. This monodispersity is essential in applications like digital PCR, single-cell analysis, or encapsulation. Explore droplet generation setup
Alginate Bead Generation In biomedical research, alginate beads are used to encapsulate cells or molecules. Pressure-driven systems allow fine-tuned control over flow rates, ensuring uniform bead size and reliable encapsulation efficiency. See the alginate bead pack.
Biology & Cell Culture
Cell Perfusion Pressure-based perfusion ensures bubble-free, pulsation-free delivery of nutrients or drugs to cultured cells over extended periods, ideal for mimicking physiological flow conditions. Discover perfusion setup
Organ-on-a-Chip Simulating organ-level responses on chips requires controlled fluid environments. Pressure-driven systems provide the accuracy and reactivity needed to replicate dynamic flow patterns and shear stress. Learn about Organ-on-a-Chip systems
Cell Confinement Assays Experiments that study how cells adapt to physical constraints benefit from stable pressure control to reproducibly apply mechanical stress. See confinement assay example
Cell Constriction Assays Revealing cellular responses to fibrotic environments or disease models requires reproducible deformation of cells, enabled by finely tuned pressure-controlled flows. View ischemia model example
Cell Trapping & Synthetic Compartments Pressure-driven flows help trap and localize cells or materials in adapted microfluidic chips, useful in synthetic biology or compartmentalization studies. Read the synthetic cells study
Flow Control & Mixin
Sequential Injection & Mixing Delivering fluids in a precise sequence or generating on-chip gradients requires fast and programmable flow transitions. Pressure controllers enable seamless transitions between multiple fluid inlets. Explore the injection pack
Recirculation Some in vitro assays require long term dynamic flow of reagents or cell culture media. In most cases it is essential to reuse the media, in order to mimic physiological conditions or avoid expenses. Using pressure-based flow control, one-way recirculation is possible to program for long term experiments. Learn about the recirculation setup
Analytical Chemistry & Imaging
Flow Chemistry Pressure-driven systems provide accurate reagent dosing and fast startup/shutdown, essential for reaction optimization in continuous-flow chemistry. This is particularly useful in the development of immunoassays and biosensors. See flow chemistry application
Liquid-Phase Electron Microscopy (LPEM) Stable, pulseless flow is critical for imaging samples in real time at the nanoscale. Pressure control allows precise delivery of fluids during live-cell imaging under an electron beam, especially to study samples in their natural environment. Explore LPEM research
Light-Field Flow Cytometry (LFC) In high-throughput single-cell analysis, stable and controlled flow conditions are key to accurate imaging and data capture. Pressure-based systems enable high-speed and consistent sample handling. Learn about 3D LFC
Industrial Applications
Enhanced Oil Recovery (EOR) Microfluidic chips simulating porous media allow researchers to visualize oil displacement mechanisms. Pressure control enables fine adjustment of flow to mimic subsurface conditions with high reproducibility. Read the full EOR review
Cosmetic Formulation & Testing
Microfluidics offers new possibilities for precision formulation of cosmetic emulsions and nano-encapsulation of active ingredients. Pressure-driven flow ensures reproducibility and scalability for testing stability, texture, or skin permeability on-chip. Explore cosmetic applications
Integrated Systems
Lab-on-a-Chip Platforms At the core of any lab-on-a-chip system is the need for precise, responsive, and stable flow control, features inherently supported by pressure-driven microfluidics. Whether it’s diagnostics, environmental sensing, or point-of-care testing, the OB1 system provides the control backbone required for success. Discover some lab-on-chip examples in this review
Videos
Publications
For all publications featuring our products, please click here .
For all application notes based on our product, please click here .
For reviews of our product, please click here .
Channel pressure range
0 to 200 mbar(1) 0 to 2,000 mbar(1) 0 to 8,000 mbar(1) -900 to 1,000 mbar(1) -900 to 6,000 mbar(1)
Pressure stability(2)
0.015 % FS(1) 0.005 % FS(1) 0.006 % FS(1) -900 to 500 mbar
-900 to 2,000 mbar
0.005 % FS(1) 0.005 % FS(1)
500 to 1,000 mbar
2,000 to 6,000 mbar
0.007 % FS(1) 0.007 % FS(1)
Response time(3)
Down to 10 ms
Setting time(4)
Down to 50 ms
Minimum pressure increment
0.006 % FS(1) 0.006 % FS(1) 0.006 % FS(1) 0.0064 % FS(1) 0.0064 % FS(1)
Pressure supply
1.5 bar (or Max pressure + 0.5 bar) to 10 bar
Input vacuum(5)
/
Any value from -0.7 to -1 bar
Input pneumatic connector
6 mm push-in
Output pneumatic connector
4 mm push-in
Liquid compatibility
Non contact pump
Flow sensor compatibility
Compatible with the whole MFS and BFS range Monitoring and feedback loop flow control available
Flow sensor compatibility
From 0,1 µL/min to 500 mL/min (indicative, please refer to the MFS and BFS series)
Flow sensor compatibility
Non-contact pump Any aqueous, oil, or biological sample solution.
Software control
Elveflow Smart Interface – Windows 10, 11, both 32 and 64 bit versions supported
Software Development Kit
Libraries available: Matlab, Python, LabView, C++
Flow sensor compatibility
Non-contact pump Any aqueous, oil, or biological sample solution. – Windows 10, 11, both 32 & 64 bit versions supported Serial/UART communication protocol on request
Data management
Possibility to log and extract data (CSV), channel and sensor detailed information using ESI
Input profiles
Possibility to load profiles: ramp, sine, triangle, square, or custom
Automation
Generate step-by-step sequences using the ESI built-in sequence management Log and export custom configurations (CSV)
Screen
LCD screen showing pressure and sensor flow rate in real time
Voltage
24V
Typical power
12W
Power connection
DC power connector, Plug, 3A, 2.1mm, 12mm
Interface
USB Type B
Communication type
Serial
Sensor connection
M8 4-pins, one connector per channel
Compatibilit
Elveflow sensors: MFS, BFS, MPS, MFP, MBD Custom sensors: 5 to 24V supply voltage, 0 to 10V readout voltage
Triggers
Input and Output TTL signal 0V or 5V
Supply Voltage Range (V)
100 to 240 VAC
Supply AC Frequency (Hz)
50 to 60 Hz
Maximum Output Current (A)
1.67 A
Maximum Output Power (W)
40 W
Dimensions (length x width x height)
240 x 223 x 83 mm
Weight
1.4 kg to 2.9 kg
(1) Max pressure value might vary by +/- 2.5% (2) Pressure stability (standard deviation) measured over the full pressure range with an external high accuracy pressure sensor (Druck DPI150) (3) Time required to reach 5% of the setting point. Depending on your computer’s operating system (4) Time required to reach 95% of the set point. Volume dependent – Measurement was done on 12 mL reservoir for a set point from 0 to 200 mbar (5) A vacuum source is mandatory for calibration and use of dual channels even if the channels are to be used in pressure only.
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[question] => What pressure range should I choose?
[reponse] =>
The performances of the OB1 (for example, the pressure stability) is tied to the full operating pressure range.
In order to achieve optimum performance, we recommend our users to choose the smallest pressure range that covers the required experimental pressures.
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[1] => Array
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[question] => Can I achieve direct flow control?
[reponse] =>
By coupling a MFS or BFS flow sensor to the OB1 flow controller, you can use the software to directly control the flow rate by directly inputting the flow rate value.
The software uses a PID loop to control the flow rate by setting automatically the pressure to reach the requested flow rate. Doing so, you can benefit of the best of the two worlds to get a fast and precise (pressure driven) volume flow rate (flow sensor).
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[2] => Array
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[question] => Can I connect a Pressure Sensor to the OB1? -
[reponse] =>
Pressure and flow sensors can be connected directly to the OB1 MK4. The sensors can be used in passive mode (for monitoring only) or active mode (to enslave).
)
[3] => Array
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[question] => Do I need a pressure and/or vacuum source to operate the OB1 Pressure controller ?
[reponse] =>
Yes, you will need a pressure source to work with the OB1 pressure controller.
You can either use the air supply of your lab if there is one, use a compressed air bottle or use an air compressor. Elveflow has selected a compressor that is perfectly fitted for most application using the OB1.
If your OB1 has a vacuum channel, you will need a vacuum pump. Elveflow also proposes a selected pump that work perfectly with the OB1.
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[question] => How to install the OB1?
[reponse] =>
Click here
Unboxing video
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[more_title] => Software
[texte] =>
Software ESI is Elveflow’s dedicated software interface, built to make life easier for experimenters. It is perfectly adapted for the control of simple and complex setups and workflow automation. It integrates several modules that make time-consuming and painful tasks simple. It comes with SDK libraries to control the Elveflow system from your own code.
The OB1 control window allows to:
Visualize the key parameters of each channel:
the command pressure
the output pressure
Visualize sensor output when one is connected to the OB1 (a flow rate sensor on the first channel in the video presented below)
Set any pressure or flow rate pattern (predefined or custom)
Easily switch to the flow rate control mode (sensor mode)
Use the pause/play button to preset the parameters on each channel and launch them simultaneously
Save configurations to gain time in the future or automate your workflow thanks to the sequencer
Open a graph to visualize and save data
Launch and use dedicated modules to simplify specific experimental tasks
Which sensor is compatible with OB1 MK4?
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[text] =>
Advantages of Pressure control in microfluidics
Fast settling time
High stability and pulseless flow
Possibility to handle fluid volumes of several liters
Enable both flow and pressure control when used with a flow meter
For more details about Pressure driven flow control, please read this application note .
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[texte_1] =>
Directly connect a flow sensor Control the flow inside your microfluidic setup
[texte_2] =>
5 pressure ranges for each channel Choose the pressure range best suited to your need
We have several pressure channel options available covering a wide range, up to 8000 mbar (116 PSI) and down to -900 mbar (-13 PSI). Choose the pressure channel that covers your experimental pressure requirements.
[texte_3] =>
Choose between 1 to 4 channels, and more… How many channels do you want?
The OB1 MK4 is a compact module that can accommodate up to 4 channels (pressure and/or vacuum). Each module can be upgraded at any time and have channels added or changed. You can control as many channels as you want with one computer.
Need more channels on one module (8, 16, 64…. or even more)? Our custom service would design a specific pressure controller that fits your needs.
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[testimonial_text] => We are using the device extensively at the moment and I have been quite impressed by its capabilities
[testimonial_author] => Dr. Hayden Taylor
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[testimonial_text] => The Elveflow OB1 enables us precise pressure and flow control and measurement in various microfluidic and lab scale projects from tissue engineering to membrane filtration analysis.
[testimonial_author] => Dr. Arne Lüken
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[testimonial_university_country_v2] => Chemical Process Engineering at RWTH Aachen
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[testimonial_text] => After an easy installation, we are now using the OB1 and flow sensors routinely and are very satisfied by both the flow stability and the software user-friendly interface.
[testimonial_author] => Dr. Nelson BC Serre & Dr. Matyáš Fendrych
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[testimonial_university_country_v2] => Cell Growth Lab, Department of Experimental Plant Biology, Charles University, Czech Republic
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[testimonial_text] => We use Elveflow instrument in several projects and they are great to work with. The LabVIEW implementation was also very successful. I hope more people can get to know the OB1 system
[testimonial_author] => Seongjin Park, Senior Engineer
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[testimonial_university_country_v2] => Department of Physics, Syracuse University
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[testimonial_text] => I have extensively used Elveflow systems in the world's most brilliant X-ray source. Elveflow systems are precise, controlled and adaptive to many samples under adverse conditions.
[testimonial_author] => Dr. Pushparani Micheal Raj
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[testimonial_university_country_v2] => Max Planck Institute of Quantum Optics, MAX IV Laboratory
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[testimonial_text] => After an easy installation, we are now using the OB1 and flow sensors routinely and are very satisfied by both the flow stability and the software's user-friendly interface.
[testimonial_author] => Nelson BC Serre and Matyáš Fendrych, Cell Growth Lab, Department of Experimental Plant Biology, Charles University, Czech Republic
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[testimonial_text] => We have used Elveflow's products for about 9 years now and are big fans, and have made lots of recommendations to friends in Wageningen and the Netherlands. They are so easy to use, reliable and the pricing is sharp. We are very happy customers!
[testimonial_author] => Prof. Dr. Joris Sprakel, Physical Chemistry and soft Matter, Wageningen university, Netherlands
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[title] => Perfect match
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[title] => 01 MFS Standard flow rate sensor ELVEFLOW MICROFLUIDICS
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Flow control systems 1 pages found
Microfluidics brings reproducibility and control over droplet size that are not achievable with any other technique.
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